Developed by Dr. Thomas Pinkerton, the result was a new phase that allows for chromatographic separations without interference by protein adsorption.
A second generation phase with an improved bonding process—bonding the GFF peptide to the silica surface through a monofunctional glycidoxypropyl linkage rather than the original trifunctional linkage.
ISPR : Internal Surface Reversed Phase.
This resulted in the following improvements:
• Increased sample retention
• Higher column efficiency
• Greater batch-to-batch reproducibility.
ISRP Selectivity
Many variables can affect the selectivity of the ISRP phase, including:
Mobile Phase Composition: The nature of ISRP analytes requires that mobile phases consist of a buffer with varying degrees of modification. Modifiers can include acetonitrile, methanol, isopropanol and tetrahydrofuran. Caution: too much modifier can result in matrix precipitation.
pH: The pH of the mobile phase can be controlled to avoid protein denaturing and to enhance selectivity. The pH range of the column is between 2.5 and 7.5; however, within the optimal pH range of 6.0 to 7.5, both the proteins and the glycine outer surface take on a negative charge. As a result, negatively charged proteins are repulsed by the outer phase and pass quickly through the column.
Temperature: Separations can also be optimized by varying column temperature. Lower temperatures have been shown to result in increased retention and selectivity.