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Pirkle Stationary Phases

Updated: Jun 18, 2021

In 1980, Regis Technologies along with Professor William Pirkle of the University of Illinois, introduced the Pirkle Chiral Stationary Phases. These Chiral Stationary Phases offer many advantages.

• Enantiomer separation on a wide variety of compound groups

• Column durability resulting from covalent phase bonding

• Ability to invert elution order

• Availability of analytical- to preparative-sized columns and bulk packing material

• Universal solvent compatibility

Enantiomer Separation

The Pirkle CSPs are covalently bonded to the silica, providing excellent column durability.

Regis manufactures 9 Pirkle CSPs. These can separate a wide variety of enantiomers in numerous compound groups. Examples include: • Aryl Propionic Acid Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) • Agricultural Compounds • Natural Products • ß-Blockers • Many Pharmaceuticals

Ability to Invert Elution Order

An important advantage of the Pirkle CSPs is the ability to invert elution order by using the same type of CSP, but with the opposite absolute configuration. As a result, it is possible to have the trace enantiomer elute before the major — a desirable feature for enantiomeric purity determinations. For preparative separations it is beneficial to elute the desired component first.

Regis Chiral Column

Chiral Purification on HPLC and SFC

Universal Solvent Compatibility Choice of mobile phase is not a limitation with the Pirkle HPLC columns. They are compatible with most mobile phases. The pH of the mobile phase, however, must be between 2.5 and 7.5. Both normal phase and reversed phase modes can be used, although normal phase is most common. For normal phase separations, the classic mobile phase is a binary or ternary mixture of a hydrocarbon and a modifier, usually an aliphatic alcohol.

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